Coding

Part:BBa_K5300007:Design

Designed by: Dini Piao   Group: iGEM24_CAU-China   (2024-09-21)


pfa2 coding region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3578
    Illegal BglII site found at 4728
    Illegal BglII site found at 5600
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 1825
    Illegal NgoMIV site found at 2382
    Illegal NgoMIV site found at 2458
    Illegal NgoMIV site found at 2742
    Illegal NgoMIV site found at 3726
    Illegal NgoMIV site found at 5700
    Illegal AgeI site found at 930
    Illegal AgeI site found at 3429
    Illegal AgeI site found at 4129
    Illegal AgeI site found at 6325
    Illegal AgeI site found at 6652
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Referencing the codon preference and GC content of rhizobia, perform codon optimization on gene clusters


Source

Aetherobacter fasciculatus (SBSr002)

References